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厌氧消化 3

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厌氧消化，生物固体，污染，甲基苯丙胺，吸附 1

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Concept and application of anaerobic suspended granular sludge bed (SGSB) reactor for wastewater treatment

Mingxia ZHENG,Zhong YAN,Jiane ZUO,Kaijun WANG

《环境科学与工程前沿（英文）》 2014年 第8卷 第5期   页码 797-804 doi: 10.1007/s11783-013-0597-x

摘要： Bed expansion serves an important function in the design and operation of an upflow anaerobic reactor. An analysis of the flow pattern of expanded granular sludge bed (EGSB) reactors shows that most EGSB reactors do not behave as expanded bed reactors, as is widely perceived. Rather, these reactors behave as fluidized bed reactors based on the classic chemical reactor theory. In this paper, four bed expansion modes, divided as static bed, expanded bed, suspended bed, and fluidized bed, for bioreactors are proposed. A high-rate anaerobic suspended granular sludge bed (SGSB) reactor was then developed. The SGSB reactor is an upflow anaerobic reactor, and its expansion degree can be easily controlled within a range to maintain the suspended status of the sludge bed by controlling upflow velocity. The results of the full-scale reactor confirmed that the use of SGSB reactors is advantageous. The full-scale SGSB reactor runs stably and achieves high COD removal efficiency (about 90%) at high loading rates (average 40 kg-COD·m ·d , maximum to 52 kg-COD·m ·d ) based on the SGSB theory, and its expansion degree is between 22% and 37%.

关键词： expansion characteristic     high-rate bioreactor     anaerobic suspended granular sludge bed     expanded granular sludge bed reactor    

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Influence of influent on anaerobic ammonium oxidation in an Expanded Granular Sludge Bed-Biological Aerated

Daijun ZHANG, Cui BAI, Ting TANG, Qing YANG

《环境科学与工程前沿（英文）》 2011年 第5卷 第2期   页码 291-297 doi: 10.1007/s11783-010-0270-6

摘要： Shortcut nitrification-denitrification, anaerobic ammonium oxidation (ANAMMOX), and methanogenesis have been successfully coupled in an Expanded Granular Sludge Bed-Biological Aerated Filter (EGSB-BAF) integrated system. As fed different synthetic wastewater with chemical oxygen demand (COD) of 300–1200 mg·L and of 30–120 mg·L at the outer recycle ratio of 200%, the influence of influent on ANAMMOX in the integrated system was investigated in this paper. The experimental results showed that higher COD concentration caused an increase in denitrification and methanogenesis but a decrease in ANAMMOX; however, when an influent with the low concentration of COD was used, the opposite changes could be observed. Higher influent concentration favored ANAMMOX when the COD concentration of influent was fixed. Therefore, low ratio would decrease competition for nitrite between ANAMMOX and denitrification, which was favorable for reducing the negative effect of organic COD on ANAMMOX. The good performance of the integrated system indicated that the bacterial community of denitrification, ANAMMOX, and methanogenesis could be dynamically maintained in the sludge of EGSB reactor for a certain range of influent.

关键词： Expanded Granular Sludge Bed-Biological Aerated Filter (EGSB-BAF) integrated system     the influence of influent     anaerobic ammonium oxidation (ANAMMOX)     shortcut nitrification-denitrification     methano-genesis    

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Effect of wastewater composition on the calcium carbonate precipitation in upflow anaerobic sludge blanket

Shucheng YANG, Yanling HE, Charles CHOU, Pengxiang ZHANG, Dongqi WANG, Yonghong LIU,

《环境科学与工程前沿（英文）》 2010年 第4卷 第2期   页码 142-149 doi: 10.1007/s11783-010-0026-3

摘要： Calcium carbonate often precipitates in anaerobic reactors treating wastewater with high calcium content. The aim of this paper is to study the effect of wastewater composition on calcium carbonate precipitation in upflow anaerobic sludge blanket (UASB) reactors. Two laboratory-scale UASB reactors were operated with calcium-containing influents using acetate and carbohydrate as substrate, respectively. There was an obvious accumulation of inorganic precipitate observed in the biogranules. Observations via scanning electron microscope (SEM) and energy dispersive spectroscopy (EDS) showed that the acclimated biogranules in the two reactors differed in microstructure. Calcium carbonate was found to have precipitated on the surface of acetate-degrading biogranules, but precipitated at the core of the carbohydrate-degrading biogranules. The results indicated that substrates had significant influence on the location of calcium carbonate precipitation in anaerobic granular sludge, which was expected due to the different methanogens distribution and pH gradient within the granular sludge degrading various substrates. Moreover, the location of calcium carbonate precipitation substantially affected the specific methanogenic activity (SMA) of the granular sludge. The SMA of the acetate-degrading biogranules dropped from 1.96 gCOD·gVSS·d to 0.61 gCOD·gVSS·d after 180-d of operation in the reactor. However, the SMA of the carbohydrate-degrading biogranules was not adversely affected by calcium carbonate precipitation.

关键词： calcium carbonate     precipitation     anaerobic granular sludge     wastewater composition     microstructure     upflow anaerobic sludge blanket (UASB)    

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Aerobic granular sludge formation based on substrate availability: Effects of flow pattern and fermentation

Quan Yuan, Hui Gong, Hao Xi, Kaijun Wang

《环境科学与工程前沿（英文）》 2020年 第14卷 第3期 doi: 10.1007/s11783-020-1226-0

摘要： Penetration depth and substrate characters affect AGS formation and performance. The relationship between substrate gradient and particle size affects AGS stability. The fermentation process is proposed as a pretreatment to improve AGS stability. The influences of flow patterns (mixed-flow and plug-flow) and fermentation pretreatment on aerobic granular sludge (AGS) formation with various substrate availability levels were investigated by running four identical laboratory-scale sequencing batch reactors (R1–R4), comparing two anaerobic feeding strategies and three kinds of substrates. R1 achieved faster granulation with a fast influent fill step followed by a modified anaerobic mixed-flow phase, but the AGS showed poorer stability with a cracked structure and a high suspended solids concentration in the effluent. The anaerobic plug-flow feeding mode (with influent fed slowly from the bottom) in R2 provided deeper penetration depth for the substance to reach the core of AGS and accordingly strengthen AGS stability. An acidogenic up-flow sludge bed reactor was introduced as a pretreatment to improve the AGS performance by enhancing glucose pre-fermentation (R4). AGS fed with mixed volatile fatty acids (VFA) after glucose fermentation showed similar performance compared with the reactor fed with acetate in the aspects of stability, structure, size distribution and nitrogen removal efficiency, and 74% similarity in the microbial community. For actual wastewater with low VFA concentrations, fermentation treatment was suggested as a promising pretreatment for stable AGS granulation and operation.

关键词： Anaerobic plug-flow feeding mode     Anaerobic mixed-flow mode     Fermentation pretreatment     Substrate gradient     Penetration depth    

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Effects of shear force on formation and properties of anoxic granular sludge in SBR

Xinyan ZHANG, Binbin WANG, Qingqing HAN, Hongmei ZHAO, Dangcong PENG

《环境科学与工程前沿（英文）》 2013年 第7卷 第6期   页码 896-905 doi: 10.1007/s11783-013-0539-7

摘要： This paper reports the effects of shear force on anoxic granular sludge in sequencing batch reactors (SBR). The study was carried out in two SBRs (SBR1 and SBR2) in which sodium acetate (200 mg COD·L ) was used as the sole substrate and sodium nitrate (40 mgNO -N·L ) was employed as the electron acceptor. The preliminary objective of this study was to cultivate anoxic granules in the SBR in order to investigate the effects of shear force on the formation of anoxic granular sludge and to compare the properties of anoxic sludge in the SBR. This study reports new results for the values of average velocity gradient, a measure of the applied shear force, which was varied in the two SBRs (3.79 s and 9.76 s for SBR1 and SBR2 respectively). The important findings of this research highlight the dual effects of shear force on anoxic granules. A low shear force can produce large anoxic granules with high activity and poor settling ability, whereas higher shear forces produce smaller granules with better settling ability and lower activity. The results of this study show that the anoxic granulation is closely related to the strength of the shear force. For high shear force, this research demonstrated that: 1) granules with smaller diameters, high density and good settling ability were formed in the reactor, and 2) granular sludge formed faster than it did in the low shear force reactor (41days versus 76 days). Once a steady-state has been achieved, the nitrate and COD removal rates were found to be 98% and 80%, respectively. For low shear force, such as was applied in SBR1, this research demonstrated that: 1) the activity of anoxic granular sludge in low shear force was higher than that in high shear force, 2) higher amount of soluble microbial products (SMPs) were produced, and 3) large pores were observed inside the larger granules, which are beneficial for nitrogen gas diffusion. Electron microscopic examination of the anoxic granules in both reactors showed that the morphology of the granules was ellipsoidal with a clear outline. Coccus and rod-shaped bacteria were wrapped by filamentous bacteria on the surface of granule.

关键词： denitrification     anoxic granular sludge     sequencing batch reactors (SBR)     shear force    

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High-solid anaerobic digestion of sewage sludge: achievements and perspectives

《环境科学与工程前沿（英文）》 2021年 第15卷 第4期 doi: 10.1007/s11783-020-1364-4

摘要：

• High-solid anaerobic digestion (HS-AD) of sewage sludge (SS) is overviewed.

关键词： High-solid effect     Anaerobic fermentation     Methane production     Biodegradability     Sludge treatment    

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Impact of roxarsone on the UASB reactor performance and its degradation

Mengchuan Shui, Feng Ji, Rui Tang, Shoujun Yuan, Xinmin Zhan, Wei Wang, Zhenhu Hu

《环境科学与工程前沿（英文）》 2016年 第10卷 第6期 doi: 10.1007/s11783-016-0871-9

摘要： Impact of continuous ROX addition on performance of UASB reactor was investigated With continuous ROX addition, severe inhibition to methanogenic activity occurred ROX addition caused the changes in the morphology and bacterial diversity of AGS A possible biotransformation pathway of ROX in the UASB reactor was proposed 60%–70% of the arsenic was discharged to the effluent, and 30%–40% was precipitated Roxarsone (3-nitro-4-hydroxyphenylarsonic acid, ROX) has been widely used for decades as an organoarsenic feed additive to control intestinal parasites and improve feed efficiency in animal production. However, most of the ROX is excreted into the manure, causing arsenic contamination in wastewater. The arsenic compounds are toxic to microorganisms, but the influence of continuous ROX loading on upflow anaerobic sludge blanket (UASB) reactor is still unknown. In this study, the impact of ROX and its degradation products on the performance of the UASB reactor and the degradation and speciation of ROX in the reactor were investigated. The UASB reactor (hydraulic retention time: 1.75 d) was operated using synthetic wastewater supplemented with ROX for a period of 260 days. With continuous ROX addition at 25.0 mg?L , severe inhibition to methanogenic activity occurred after 87 days operation accompanied with an accumulation of volatile fatty acids (VFAs) and a decline in pH. The decrease of added ROX concentration to 13.2 mg?L did not mediate the inhibition. As(III), As(V), MMA(V), DMA(V), HAPA and an unknown arsenic compound were detected in the reactor, and a possible biotransformation pathway of ROX was proposed. Mass balance analysis of arsenic indicated that 60%–70% of the arsenic was discharged into the effluent, and 30%–40% was precipitated in the reactor. The results from this study suggest that we need to pay attention to the stability in the UASB reactors treating organoarsenic-contaminated manure and wastewater, and the effluent and sludge from the reactor to avoid diffusion of arsenic contamination.

关键词： Anaerobic digestion     Anaerobic granular sludge (AGS)     Arsenic species     Impact     Roxarsone (ROX)     UASB reactor    

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Cultivation of aerobic granular sludge in a conventional, continuous flow, completely mixed activatedsludge system

Xi CHEN,Linjiang YUAN,Wenjuan LU,Yuyou LI,Pei LIU,Kun NIE

《环境科学与工程前沿（英文）》 2015年 第9卷 第2期   页码 324-333 doi: 10.1007/s11783-014-0627-3

摘要： Aerobic granules were formed in a conventional, continuous flow, completely mixed activated sludge system (CMAS). The reactor was inoculated with seed sludge containing few filaments and fed with synthetic municipal wastewater. The settling time of the sludge and the average dissolved oxygen (DO) of the reactor were 2 h and 4.2 mg·L , respectively. The reactor was agitated by a stirrer, with a speed of 250 r·min , to ensure good mixing．The granular sludge had good settleability, and the sludge volume index (SVI) was between 50 and 90 mL·g . The laser particle analyzer showed the diameter of the granules to be between 0.18 and 1.25 mm. A scanning electron microscope (SEM) investigation revealed the predominance of sphere-like and rod-like bacteria, and only few filaments grew in the granules. The microbial community structure of the granules was also analyzed by polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE). Sequencing analysis indicated the dominant species were α, β, and γ- , , and . The data from the study suggested that aerobic granules could form, if provided with sufficient number of filaments and high shear force. It was also observed that a high height-to-diameter ratio of the reactor and short settling time were not essential for the formation of aerobic granular sludge.

关键词： aerobic granular sludge     completely mixed activated sludge system (CMAS)     continuous flow     shear force     filamentous bacteria     polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE)    

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Evaluating the impact of sulfamethoxazole on hydrogen production during dark anaerobic sludge fermentation

《环境科学与工程前沿（英文）》 2023年 第17卷 第1期 doi: 10.1007/s11783-023-1607-2

摘要：

● SMX promotes hydrogen production from dark anaerobic sludge fermentation.

关键词： Sulfamethoxazole     Hydrogen production     Dark anaerobic fermentation     Waste activated sludge    

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Achievement of high rate nitritation with aerobic granular sludge reactors enhanced by sludge recirculation

Zulkifly JEMAAT,Josep Anton TORA,Albert BARTROLI,Julián CARRERA,Julio PEREZ

《环境科学与工程前沿（英文）》 2015年 第9卷 第3期   页码 528-533 doi: 10.1007/s11783-014-0641-5

摘要： A ratio control strategy has been used to demonstrate the feasibility of this automatic control procedure for the achievement of stable full and partial nitritation. The control strategy assured constant ratio between the dissolved oxygen (DO) and the total ammonia nitrogen (TAN) concentrations in the bulk liquid of aerobic granular sludge reactors operating in continuous mode. Three different set-ups with different reactor capacities were used (3, 110, and 150 L). High strength synthetic wastewaters and reject water were tested with similar performance. Achieved nitrogen loading rates ranged between 0.4 and 6.1 kgN·m ·d , at temperatures between 20°C and 30°C. Granular sludge and nitritation were stable in the long term continuous operation of the reactors. Suitable stable effluent for Anammox has been obtained using the desired TAN setpoint (i.e. 50% of influent ammonium oxidation). An existing biofilm model developed incorporating the implemented control loops and validated in a previous publication was used to investigate the effects of the ammonium concentration of the influent and the biofilm density on the achievement of full nitritation. The model demonstrated how sludge recirculation events led to a stable and significant increase of the biomass concentration in the reactor, which in turn resulted in the achievement of high nitrogen loading rates, due to the action of the control strategy. The model predicted an enhancement of stable full nitritation at higher ammonium concentrations in the influent. Poor influence of the biofilm density in the achievement of full nitritation was predicted with the model.

关键词： partial nitrification     reject water     high strength ammonium wastewater     closed-loop control    

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Biosorption of Direct Black 38 by dried anaerobic granular sludge

WANG Xuejiang, XIA Siqing, ZHAO Jianfu

《环境科学与工程前沿（英文）》 2008年 第2卷 第2期   页码 198-202 doi: 10.1007/s11783-008-0031-y

摘要： The biosorption of Direct Black 38 by dried anaerobic granular sludge in a batch system under specific temperatures and initial pH was investigated. The adsorption reaction is pH dependent with higher removal at low pH. The adsorption equilibrium data fit very well with both Langmuir and Freundlich models in the concentration range of Direct Black 38 at all chosen temperatures. The adsorption parameters show that the adsorption of Direct Black 38 is an endothermic and more effective process at high temperatures. The kinetics of adsorption was found to be second order and adsorption rate constants increased with increasing temperature. Activation energy was determined as 26.8 kJ/mol for the process. This suggests that the adsorption of Direct Black 38 by dried anaerobic granular sludge is chemically controlled.

关键词： Langmuir     kJ/mol     temperature     removal     Freundlich    

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Influencing mechanism of high solid concentration on anaerobic mono-digestion of sewage sludge without

Yuyao ZHANG,Huan LI,Can LIU,Yingchao CHENG

《环境科学与工程前沿（英文）》 2015年 第9卷 第6期   页码 1108-1116 doi: 10.1007/s11783-015-0806-x

摘要： High-solids anaerobic digestion of sewage sludge was a promising process, but high solid concentration negatively influenced methane production. The influencing mechanism was systematically analyzed in this study through a series of static anaerobic digestion experiments at total solids (TS) contents of 3%–15%. The results showed that TS 6% was the boundary between low-solids and high-solids anaerobic digestion, and the accumulative methane yield decreased exponentially when TS increased from 6% to 15%. The performance of anaerobic digestion was directly determined by the efficiency of mass transfer, and the relation between methane yield and sludge diffusive coefficients was well described by a power function. Thus, the increasing TS resulted in an exponential increase in sludge viscosity but an exponential decrease in diffusive coefficient. The blocked mass transfer led to the accumulation of volatile fatty acids (VFAs) and free ammonia. Acetic metabolism was the main process, whereas butyric and propionic metabolisms occurred at the initial stage of high-solids anaerobic digestion. The concentration of VFAs reached the maximum at the initial stage, which were still lower than the threshold influencing methanogens. The concentration of free ammonia increased gradually, and the methanogenesis was inhibited when free ammonia nitrogen exceeded 50 mg·L . Consequently, the deterioration of high-solids anaerobic digestion was related to the blocked mass transfer and the resulting ammonia accumulation.

关键词： anaerobic digestion     methane     sewage sludge     volatile fatty acids     free ammonia    

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Structure and formation of anoxic granular sludge —A string-bag hypothesis

Binbin WANG,Dangcong PENG,Xinyan ZHANG,Xiaochang WANG

《环境科学与工程前沿（英文）》 2016年 第10卷 第2期   页码 311-318 doi: 10.1007/s11783-014-0748-8

摘要： Anoxic granular sludge was developed in a laboratory-scale sequencing batch reactor which was fed with sodium acetate and sodium nitrate as electron donor and accepter. The sludge in the reactor was almost granulated after approximately 90 days of cultivation. In the present study, a detailed examination of surface morphology and internal structure of anoxic granular sludge was conducted using scanning electron microscope. It showed that the bacteria inside the granules had a uniform, coccus-like shape. By contrast, filamentous bacteria were predominant outside the granules. These bacteria were woven and had wrapped the coccus bacteria together to form granules. The small amounts of DO in the liquid bulk promoted the growth of filamentous bacteria on the surface of the granules. A string-bag hypothesis was proposed to elucidate the structure and formation of the anoxic granular sludge. It suggested that micro-aeration could be a method to promote granulation in practical anoxic treatment systems.

关键词： granulation     sequencing batch reactor     anoxic sludge     scanning electron microscope     filamentous bacteria    

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Distribution and removal of antibiotic resistance genes during anaerobic sludge digestion with alkaline

Mengli Wang, Ruying Li, Qing Zhao

《环境科学与工程前沿（英文）》 2019年 第13卷 第3期 doi: 10.1007/s11783-019-1127-2

摘要：

Sludge digestion is critical to control the spread of ARGs from wastewater to soil.

Fate of ARGs in three pretreatment-AD processes was investigated.

UP was more efficient for ARGs removal than AP and THP in pretreatment-AD process.

The total ARGs concentration showed significant correlation with 16S rRNA gene.

The bacteria carrying ARGs could be mainly affiliated with Proteobacteria.

关键词： Tetracycline resistance genes     Sulfonamide resistance genes     16S rRNA gene     Sludge pretreatment     Anaerobic sludge digestion    

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Tank-dependence of the functionality and network differentiation of activated sludge community in a full-scaleanaerobic/anoxic/aerobic municipal sewage treatment plant

《环境科学与工程前沿（英文）》 2023年 第17卷 第3期 doi: 10.1007/s11783-023-1636-x

摘要：

● Environmental parameters affected functional bacteria and network associations.

关键词： Activated sludge     Bacterial community     Tank-dependence     Network association     Functional bacteria    

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标题 作者 时间 类型 操作

Concept and application of anaerobic suspended granular sludge bed (SGSB) reactor for wastewater treatment

Mingxia ZHENG,Zhong YAN,Jiane ZUO,Kaijun WANG

期刊论文

Influence of influent on anaerobic ammonium oxidation in an Expanded Granular Sludge Bed-Biological Aerated

Daijun ZHANG, Cui BAI, Ting TANG, Qing YANG

期刊论文

Effect of wastewater composition on the calcium carbonate precipitation in upflow anaerobic sludge blanket

Shucheng YANG, Yanling HE, Charles CHOU, Pengxiang ZHANG, Dongqi WANG, Yonghong LIU,

期刊论文

Aerobic granular sludge formation based on substrate availability: Effects of flow pattern and fermentation

Quan Yuan, Hui Gong, Hao Xi, Kaijun Wang

期刊论文

Effects of shear force on formation and properties of anoxic granular sludge in SBR

Xinyan ZHANG, Binbin WANG, Qingqing HAN, Hongmei ZHAO, Dangcong PENG

期刊论文

High-solid anaerobic digestion of sewage sludge: achievements and perspectives

期刊论文

Impact of roxarsone on the UASB reactor performance and its degradation

Mengchuan Shui, Feng Ji, Rui Tang, Shoujun Yuan, Xinmin Zhan, Wei Wang, Zhenhu Hu

期刊论文

Cultivation of aerobic granular sludge in a conventional, continuous flow, completely mixed activatedsludge system

Xi CHEN,Linjiang YUAN,Wenjuan LU,Yuyou LI,Pei LIU,Kun NIE

期刊论文

Evaluating the impact of sulfamethoxazole on hydrogen production during dark anaerobic sludge fermentation

期刊论文

Achievement of high rate nitritation with aerobic granular sludge reactors enhanced by sludge recirculation

Zulkifly JEMAAT,Josep Anton TORA,Albert BARTROLI,Julián CARRERA,Julio PEREZ

期刊论文

Biosorption of Direct Black 38 by dried anaerobic granular sludge

WANG Xuejiang, XIA Siqing, ZHAO Jianfu

期刊论文

Influencing mechanism of high solid concentration on anaerobic mono-digestion of sewage sludge without

Yuyao ZHANG,Huan LI,Can LIU,Yingchao CHENG

期刊论文

Structure and formation of anoxic granular sludge —A string-bag hypothesis

Binbin WANG,Dangcong PENG,Xinyan ZHANG,Xiaochang WANG

期刊论文

Distribution and removal of antibiotic resistance genes during anaerobic sludge digestion with alkaline

Mengli Wang, Ruying Li, Qing Zhao

期刊论文

Tank-dependence of the functionality and network differentiation of activated sludge community in a full-scaleanaerobic/anoxic/aerobic municipal sewage treatment plant

期刊论文